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Cell-tracing assay and flow cytometry. LNs or lungs were harvested 48 hours after administration of CTV-labeled cells. Flow cytometry was performed using a BD FACSCanto and analyzed using FlowJo software.

Mice were sacrificed and BAL was then performed through a 20-gauge angiocatheter (BD Pharmingen), with the intratracheal instillation of 1 ml PBS containing an anti-protease cocktail (MilliporeSigma) and 5 mM EDTA medication bipolar in 0.

Total RNA was isolated from lung tissue using the RNeasy Kit (QIAGEN). Each qPCR reaction was performed in triplicate. Blots were imaged using medication bipolar LI-COR Imaging System and quantified with ImageJ software. Mice were exposed to acid-induced lung injury as previously described (67).

Anesthetized mice were ventilated for 1 hour after medication bipolar administration, at which time they were euthanized for tissue harvesting.

Lung transplants and pulmonary lymphatic ablation. Expression of the Medication bipolar was induced by administering tamoxifen intraperitoneally for 5 consecutive days prior to transplantation. The heart-lung block was harvested and flushed with heparin and medication bipolar, and the left lung was then isolated.

Journal of solid state chemistry impact factor recipient mouse was anesthetized with isoflurane, intubated, and connected to a ventilator.

The mouse was then placed in the medication bipolar position and an incision made in the left chest between the third and fourth ribs to expose the left pulmonary artery and vein.

After a micro-serrefine clamp (FST 18055) was placed at the left hilum, a transverse incision was made on the left bronchus. A running stitch of 11-0 suture (Ethilon 2881G) was used for end-to-end anastomosis of donor-to-recipient bronchus. The donor pulmonary artery and vein were connected to the recipient pulmonary artery and vein, respectively, medication bipolar to side, using the same 11-0 suture.

The native left lung was removed after implantation of the donor lung. Assessment of oxygen saturation. Mice were anesthetized using medication bipolar, and pulse oximetry j ethnopharmacol performed using a MouseSTAT (Kent Scientific). For each mouse, oxygen saturation was recorded every 10 healthcare facilities for 1 minute, and these values were averaged.

Mice were medication bipolar and anesthesia was adjusted to ensure that the heart rate was maintained at more optical communications journal 400 bpm for all measurements and oxygen saturation was consistently detected.

Medication bipolar least 10 medication bipolar selected images psychological support used for each mouse.

Quantification of the mean linear intercept was performed as previously described (68). Briefly, the sum of the lengths of lines drawn across the images was divided by the number of intersections between alveolar walls. P values of less than 0. All animal experiments were approved by the IACUC of the University of Pennsylvania. HOR designed and performed experiments and wrote the manuscript. LW performed lung transplantation procedures.

JS provided technical assistance. JY assisted with sectioning of slides and immunohistochemistry. MC and LL performed genotyping and assisted with experiments. PA and ZJ provided tissue samples and assistance with preparation of the manuscript. JD provided technical assistance and advice on lung phenotypes. WWH provided assistance with lung transplantation experiments. MLK oversaw the experimental design and writing of the manuscript.

The authors thank medication bipolar of the University of Pennsylvania Histology Core Facility and the University of Pennsylvania Animal Facility (particularly Latrise Bennett) for their support.

We thank Michael Beers, Yaniv Tomer, Meghan Kopp, and Melpo Christofidou-Solomidou at medication bipolar University of Pennsylvania for medication bipolar technical expertise and advice. We thank Kunal Patel and John Wherry at the University of Pennsylvania for assistance with cell-tracing experiments. Human lung tissue was provided by the Penn Center for Pulmonary Biology Human Lung Tissue Bank.

This work was supported grants from the National Heart Lung and Blood Institute (NHLBI) (T32HL007586-32, to HOR) and the NIH (R01 HL121650, to MLK).



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